Restructuring Tilth Layers Can Change the Microbial Community Structure and Affect the Occurrence of Verticillium Wilt in Cotton Field

Restructuring tilth layers (RTL) is a tillage method that exchanges the 0–20 and 20–40 cm soil can be applied during cotton cultivation to increase yield, eliminate weeds alleviate severe disease, including Verticillium wilt. However, mechanism by which RTL inhibits wilt unclear. Therefore, we investigated distribution of microbial communities after rotary (CK) treatments identify reasons for reduction in fields subjected RTL. Illumina high-throughput sequencing was used sequence bacterial fungal genes. The disease incidence severity decreased 28.57% 42.64%, respectively, Moreover, significantly enhanced richness evenness at -reduced differences between depths cm. number Verticillium dahliae decreased, while relative abundance biocontrol bacteria such as Bacillus Pseudoxanthomonas increased following Overall improved diversity, bacteria, may have suppressed occurrence fields.